PuraMatrix® is a transparent, biocompatible, synthetic matrix that is used to create defined three-dimensional microenvironments for a variety of cell culture experiments. To achieve optimal cell growth and differentiation, it is necessary to determine the appropriate mixture of this material and bioactive molecules (e.g., growth factors, extracellular matrix [ECM] proteins, and/or other molecules).

PuraMatrix® consists of standard amino acids (1% w/v) and 99% water. Under physiological conditions, the peptide component self-assembles into a 3-D hydrogel that exhibits a nanometer scale fibrous structure (Figure at the right). The hydrogel is readily formed in a culture dish, plate, or cell culture insert.

PuraMatrix® Synthetic Extracellular Matrix

Characteristics

  • PuraMatrix® promotes cell attachment, but does not activate RGD-dependent integrin signalling
  • In the presence of salt-containing solution, the peptide component of PuraMatrix® self-assembles and forms a transparent 3D hydrogel
  • Exhibits nanometer scale fibrous structure
  • Biocompatible; devoid of animal-derived material and pathogens

Technical Specifications

  • 1% solution (w/v) of purified synthetic peptide
  • Packaged material exhibits pH = 3.0
  • Quality Control:
    • Tested and found negative for bacteria, fungi, and Mycoplasma
    • Cell viability > 80% based on cytotoxicity analysis of NIH3T3 fibroblasts
    • Identity confirmed using Mass Spectrometry
    • Demonstration of fiber formation using a self-assembly assay